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Comparison of Different Methods To Isolate High Molecular Weight DNA From Bacteria

The first step in many sequencing experiments is to isolate nucleic acids from a specimen, and many commercial companies now offer kits specific to the extraction of high molecular weight (HMW) genomic DNA (gDNA). However, the size and quality of gDNA can vary greatly depending upon the extraction method used. 

Download this app note to find out how scientists determined which of five extraction methods was optimal for use in a long-read sequencing workflow by comparing the:

  • Sample quality
  • Sample size
  • Sequencing results