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Detecting Adapter Dimers in NGS Libraries

 DNA fragments
Credit : Istock

Adapters play a crucial role in next-generation sequencing (NGS). They enable DNA fragment binding to flow cell hardware and can be used to identify sequences in multiplexed library analysis. However, adapters can be prone to dimerization, which can result in inaccurate data, decreased quantification precision, suboptimal flow cell loading and compromised genome coverage.

This technical overview highlights the latest fragment analysis and electrophoresis solutions for adapter dimer detection. It explores how greater detection and resolution capabilities can support sequencing efficiency and quality control (QC).

Download this technical note to discover:

  • The crucial role of adapters dimers in NGS library preparation workflows
  • A preoptimized method for adapter dimer detection
  • Ways to improve QC and decision making in your NGS applications

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