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Discover the Freshness of Frozen PBMCs

Discover the Freshness of Frozen PBMCs content piece image

Human peripheral blood mononuclear cells (PBMCs) are used to determine whether a drug will elicit a T-cell response to a foreign substance as they consist of a mixed population of specialized immune cells. PBMCs are routinely isolated and frozen down for experimental use, but what impact does cryopreservation have on these cells?

Download this app note to discover the experimental methods used to determine that: 

  • PBMCs isolated from fresh and cryopreserved leukopaks maintain function
  • PBMCs isolated from both types of starting material are reliable, reproducible and interchangeable
  • Cryopreservation has no impact on the isolation of PBMCs