Evaluation of Recombinase Polymerase Amplification (RPA) Volume Tolerance

DNA/RNA amplification performed by PCR or isothermal methods operating above 50˚C is often restricted to reaction volumes in the range of 10-100μl, and may require oil overlays or similar containment strategies. This is because, on the one hand, evaporation can limit small reaction volume formats, while on the other hand use of larger reaction volumes can complicate the consumable design required for efficient thermal control during temperature cycling event (or if isothermal, require longer to reach temperature). TwistDx’s proprietary Recombinase Polymerase Amplification (RPA) method addresses these challenges.