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Focus on Multiplexing using Recombinase Polymerase Amplification (RPA)

RPA is an isothermal nucleic acid amplification technology which works with a single primer pair, per target similar to PCR. A number of alternative isothermal methods, including LAMP, require more than two primers per target. As only two primers are required for RPA, multiplexing is relatively simple. Multiplexing is the simultaneous
amplification of more than one target in a single reaction. The primers used for the
amplification of each of the multiplexed targets have to be tested and potentially optimised for compatibility.