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Maximize Your Long-Read Sequencing Results

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Comparison of Agilent Femto Pulse System Sizing With Long-Read Sequencing Read Length 

Routine, robust methods for long-read sequencing have the potential to allow researchers to analyze long stretches of DNA without the requirement of preamplification and can also enable analysis of complex regions that are otherwise difficult to map.  

While long-read sequencing methods provide a number of advantages over short-read NGS approaches, they can be challenging due to the preferential sequencing of short fragments. One strategy to address this is size selection of the sample, allowing the sequenced fragment lengths to be maximized by selectively loading long fragments onto the sequencer. 

Download this app note to discover a system that can: 

  • Assess the quality of the size-selected DNA
  • Confirm that the fragments below the threshold size were successfully eliminated
  • Help determine appropriate size-selection thresholds and aid in quality assessment
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