Multiple Focused CRISPR Libraries From a Single Oligo Pool
Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries. Fully custom sgRNA libraries solve this issue by allowing tunability and flexibility in a CRISPR screen.
Download this app note to discover a custom sgRNA library design that:
- Can be split into multiple sub-pools with ease, each with tightly uniform distribution
- Can be used for focused genomic interrogations without wasted screening, saving time and money