Optimized cell culture
Application Note Feb 27, 2014
Tecan Group Ltd.
Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
In addition to cell culture flasks (T-Flask, RoboFlask™), cells may be grown in multiwell plates – such as 6-well, 12-well and 24-well plates.
This application note describes an automated procedure for harvesting commonly used human and animal cell lines that have been cultivated in multiwell plates, relieving laboratory personnel from tedious cell harvesting work and providing more time for other valuable tasks.