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Optimizing RNA Sequencing of FFPE Samples

RNA
Credit: iStock

Formalin-fixed paraffin-embedded (FFPE) samples held in clinical laboratories are an invaluable resource for translational research, especially in the era of personalized medicine.

However, FFPE processing and tissue storage are known to cause sample degradation, resulting in low sample input and ultimately limiting gene expression-based biomarker discovery using RNA sequencing. As a result, samples need to be enriched for mature mRNAs by depleting highly abundant ribosomal RNAs (rRNAs) before sequencing can occur.

This application note evaluates several commercially available rRNA depletion kits, providing an optimized protocol for the efficient removal of rRNA from FFPE samples. 

Download this app note to ensure:

  • Superior sequencing data from degraded RNA, including FFPE-derived RNA
  • A streamlined workflow that generates high-quality libraries 
  • Improved reproducibility and consistency in RNA sequencing results


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