Rapid Development of a 9 component Multivariate Calibration for the analysis of Metabolites in Chemically Defined Cell Culture Media
This study assessed the performance of the HyperFlux™ PRO Plus Raman Spectroscopy System
(Tornado Spectral Systems) for the quantitative analysis of biochemical components in a
simplified chemically defined pseudo growth medium for mammalian cell culture. An experiment
was designed to evaluate the ability of Raman Spectroscopy to directly measure individual
components in a complex mixture at concentrations at or below the limit of quantification of
conventional Raman spectrometers. A set of samples with varying amounts of glucose, lactate,
glutamine, glutamate, ammonium, arginine, histidine, leucine, and phenylalanine were prepared
so that covariance between components was close to zero. The spectral collection and model
development were completed in one day. The sample spectra were collected in a morning using
fast acquisition times and promising calibrations were developed in the afternoon using basic
pretreatments such as derivatives and normalisation.
(Tornado Spectral Systems) for the quantitative analysis of biochemical components in a
simplified chemically defined pseudo growth medium for mammalian cell culture. An experiment
was designed to evaluate the ability of Raman Spectroscopy to directly measure individual
components in a complex mixture at concentrations at or below the limit of quantification of
conventional Raman spectrometers. A set of samples with varying amounts of glucose, lactate,
glutamine, glutamate, ammonium, arginine, histidine, leucine, and phenylalanine were prepared
so that covariance between components was close to zero. The spectral collection and model
development were completed in one day. The sample spectra were collected in a morning using
fast acquisition times and promising calibrations were developed in the afternoon using basic
pretreatments such as derivatives and normalisation.
