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Sample Evaporation: A Comparison of Several Thermal Cycler Models

Reaction evaporation is a common occurrence, and it can often be a significant factor in determining the success or failure of PCR. Evaporation can lead to a change in pH, an increase in salt concentration, and a decrease in thermal mass. Such a change in reaction chemical composition has the potential to alter the uniformity and robustness of amplification, which in many cases is critical for the correct analysis and interpretation of experimental data.

Download this app note to discover thermal cycler systems that:

  • Demonstrate low evaporation
  • Perform consistently despite the plastics and volumes used
  • Maintain the uniformity and robustness of amplification