Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Library Preparation

After the completion of sequencing the human genome in 2003, it became obvious that novel methods would have to be developed to sequence multiple genomes of diverse species including our own. Since then, strategies for massive parallel sequencing have revolutionized research across diverse scientific disciplines. Despite these advances, DNA and RNA sample preparations, one of the most important aspects of next generation sequencing, continue to use out-dated and cumbersome methods. Current fragmenta-tion, ligation and amplification methods are susceptible to sequence bias, require significant preparatory time and are highly inefficient. Generating high quality NGS data begins with high quality libraries that have the desired insert size and proper adapter liga-tion.