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Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital PCR

Genome editing has become a widely used tool in research and drug discovery. However, the often low frequency of gene edits hinders reliable detection by methods gel- or sequencing-based methods, or requires laborious clonal isolation of edited cells.

In this article we describe an ultra-sensitive, cost-efficient Droplet Digital PCR–based method for the detection of HDR and NHEJ alleles generated using the CRISPR/Cas9 and TALEN genome editing systems.