Western Blotting is a tried and tested, widely used technique by researchers to separate, detect and analyze proteins. The method is based on building an antibody-protein complex via the specific binding of antibodies to proteins immobilized on a membrane. First described in 1979, Western Blotting continues to be a powerful tool for scientists across a variety of applications including life science research and diagnostics.
Whether you are a beginner or an expert, this selection of guides will help you not only discover the technique step-by-step but also how to optimize methods, as well as choose and maximize the use of your imaging systems along with useful hits and tips.
Access this compendium to explore:
- Western blotting - principle and methods
- Seven-step guide to western blotting
- Innovative solutions for accurate western blot imaging
Save time in western blotting How much time do you spend finding the right exposure settings for Western blots? Find out how the right CCD imager can help you work more efficiently, freeing valuable resources to drive your research forward, faster. Western blotting efficiency in the lab Using time in the lab efficiently promotes success in research with faster generation of key data leading to scientific breakthroughs. To drive efficiency, it is important to watch for ways to improve and refine routine practices and procedures, as time savings in these daily repetitive activities can add up quickly. Western blotting is an affordable process that reliably answers important basic questions regarding proteins of interest. For this reason, Western blots, or protein immunoblots, are used daily by most labs working on protein-based research. While the Western blotting method has stayed largely unchanged over the last few decades, there remains opportunity to increase efficiency in the process. Saving even just a little bit of time on every Western blot can add up to substantial time-savings over the course of a week, month, or year—time you and your colleagues can spend on other valuable research efforts. Optimizing exposure times in CCD imaging Western blots often have bands of varying intensity, with low-expression proteins presenting with lower band intensity and highly expressed proteins represented by more intense bands. Low-intensity bands need longer exposure times to be visible,