Multiplexed Assays for Diagnostics and Environment
Conference Recording Apr 28, 2014
About the SpeakerLoïc J. Blum received the Doctorat de spécialité (1983) in Biochemistry and the Doctorat d’Etat ès Sciences (1991) from Lyon 1 University. He is presently full Professor of Biochemistry and Biotechnology at the same university and is involved in the development biosensors, bioanalytical micro and nano systems, biochips and biomimetic membranes. AbstractA multiplexed blood group genotyping system based on a classical 96-well plate with up to 200 spots per well was first developed associated with a colorimetric detection. An innovative immobilization process of biological elements on standard polymeric adhesive supports applicable to 96-, 384- and 1536-well micro-plates has been developed. This technology was successfully transferred to protein immobilization for multiplex allergy diagnosis. For that purpose, crude allergen extracts and recombinant proteins (birch tree pollen, hazelnut, cherry, apple) were arrayed for the detection of allergen-specific IgE in crude human sera. Another application concerns the simultaneous detection of five water pollutants (okadaic acid; atrazine; 2.4-dichlorophenoxyacetic acid; 2,4,6-trinitrotoluene and 1,3,5-trinitroperhydro-1,3,5-triazine).Using a double role membrane for both bioprobe immobilization and reaction media filtering, tumor-associated antigens microarrays were also designed to detect auto-antibodies directly in patient sera. For that application, 12 different probes were selected according to their occurrence in cancer pathologies.