The Impact of Ligation Bias on Plant Small RNA Sequencing
Conference Recording Nov 13, 2013
About the SpeakerTamas Dalmay graduated from the Corvinus University, Budapest in 1990 and later obtained his PhD on molecular virology from the Agricultural Biotechnology Centre, Hungary. He spent seven years in David Baulcombe’s lab at the Sainsbury Laboratory (Norwich, UK) as a postdoctoral researcher. During this period he identified several genes required for transgene silencing in Arabidopsis. In 2002 he started his own group at the School of Biological Sciences at the University of East Anglia (Norwich). The last ten years his group investigated various aspects of regulatory small RNAs in fungi, plants and animal/human cells.
AbstractNext generation sequencing became the most popular approach to profile the expression of small non-coding RNAs (sRNAs). However, cDNA libraries of sRNAs are biased and this is based on the ability of individual sRNAs to form stable secondary structures with the adapters. We demonstrated that adding degenerated nucleotides to the adapters reduce the ligation bias by allowing different sequences to form a stable structure with the adapters. The impact of this ligation bias on sequencing plant sRNAs will be presented.