How To Beat Bias in Directed Evolution of Biocatalysts

Directed evolution improves the function of proteins and enzymes, and has applications in organic synthesis and biotechnology. However, multiple shortcomings prevent researchers from using this approach to its full potential. Because of problems in common PCR-based mutagenesis techniques, there is an urgent need to develop efficient screening and selection tools for biocatalyst improvement.

Scientists can mitigate PCR difficulties by using solid-phase DNA synthesis technology for combinatorial variant library generation. This approach reduces codon bias, avoids sequence errors, and allows for the identification of novel mutants with improved properties.

Download this app note to discover a novel approach using solid-phase synthesis of oligonucleotides that can:

• Reduce codon bias and sequence errors  
• Identify novel mutants with improved properties
• Accelerate the discovery of proteins with enhanced properties and functionalities