8 Steps to Successful SMRT Sequencing
How To Guide Oct 31, 2018 | by Nick Sisneros, Director – North America FAS, Pacific Biosciences
When Single Molecule, Real-Time (SMRT) sequencing was first released, it promised a huge shift in our approach to sequencing – moving away from methods that had up to that point been validated and entrenched in labs across the world. In this guide, PacBio's Nick Sisneros provides some insight into SMRT Sequencing and the tips and tricks that will ensure success in the template prep and sample preparation for use on this sequencing platform.
Download this guide to learn:
- The steps involved in a SMRT assay, from sample QC to analysis
- The importance of sample prep to a successful SMRT assay
- Expert tips for success
RELATED HOW TO GUIDES
Ribonucleic Acid (RNA) is essential for genetic regulation and expression. In many neuroscience studies, animal models such as mice are used, and accordingly, you may find that you must extract RNA from mouse brain tissue at some point or other.
There are unique challenges that arise when trying to extract RNA from mouse brain samples. This guide outlines the main precautions you should take to overcome these challenges and achieve successful RNA extraction.