How To Create a shRNA Knockdown

Shortly after the cellular mechanism of RNA interference (RNAi) was first described, scientists began utilizing this powerful technique to study gene function. The most effective way to alter a gene’s function is through sequence-specific gene silencing.

One RNAi-based method to silence a gene is through the delivery of artificial RNA molecules called short hairpin RNAs (shRNAs). This guide will focus on the design, delivery and use of shRNAs to create a long-term knockdown of a targeted gene.

Download this guide to discover:

• A full protocol for shRNA knockdown
• Diagrams explaining primer design and colony screening
• Tips and tricks on optimizing your shRNA workflows