We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.


How To Create a shRNA Knockdown

How To Create a shRNA Knockdown content piece image

Shortly after the cellular mechanism of RNA interference (RNAi) was first described, scientists began utilizing this powerful technique to study gene function. The most effective way to alter a gene’s function is through sequence-specific gene silencing.

One RNAi-based method to silence a gene is through the delivery of artificial RNA molecules called short hairpin RNAs (shRNAs). This guide will focus on the design, delivery and use of shRNAs to create a long-term knockdown of a targeted gene.

Download this guide to discover:

  • A full protocol for shRNA knockdown
  • Diagrams explaining primer design and colony screening
  • Tips and tricks on optimizing your shRNA workflows