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How To Create a shRNA Knockdown
How To Guide

How To Create a shRNA Knockdown

How To Create a shRNA Knockdown
How To Guide

How To Create a shRNA Knockdown

Shortly after the cellular mechanism of RNA interference (RNAi) was first described, scientists began utilizing this powerful technique to study gene function. The most effective way to alter a gene’s function is through sequence-specific gene silencing.


One RNAi-based method to silence a gene is through the delivery of artificial RNA molecules called short hairpin RNAs (shRNAs). This guide will focus on the design, delivery and use of shRNAs to create a long-term knockdown of a targeted gene.


Download this guide to discover:

  • A full protocol for shRNA knockdown
  • Diagrams explaining primer design and colony screening
  • Tips and tricks on optimizing your shRNA workflows
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