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Navigating Hurdles in Achieving HPLC Reproducibility

Hands loading samples into a tray for HPLC analysis.
Credit: iStock

High-performance liquid chromatography (HPLC) is a common analytical technique and plays a pivotal role in separating, identifying and quantifying complex samples. Consequently, it has found applications in diverse fields including academic research, biotechnology, the chemical industry, environmental sciences and pharmaceutical, food and beverage testing.


However, operational challenges in HPLC are common, including issues like poor reproducibility, poor peak shape, instrument sensitivity and detector drift.


Download this guide to explore tips on: 
  • Sample preparation and quality control
  • Column selection and care
  • Method development, validation and verification

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