7 Tips to Improve Your Western Blot
List Jun 22, 2018 | by Karishma Joshi, 2nd Year PhD Student, Department of Neuroscience, Psychology & Behaviour, The University of Leicester, UK
How hard can it be to get a nice single band on a clean blot? The answer, VERY!
Follow these 7 top tips to improve your westerns and reap the rewards of clean bands and publishable quality data, every time.
This how-to guide gives you practical advice on:
- Cleaning your equipment
- Making your best gel
- Setting up electrodes
- Transferring proteins from gel to membrane
- Choosing the right antibody
- Testing your antibodies
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