454 Sequencing Helps Identify Herpes-like Viruses that may Contribute to Pandemic Decline of Tropical Reefs
News Feb 05, 2009
In an effort to determine the influence of viral pathogens in declining coral health, researchers at San Diego State University recently used metagenomic analysis with the Genome Sequencer System from 454 Life Sciences to sequence Porites compressa coral samples under varying environmental stressors.
The purpose of the study, led by Dr. Forest Rohwer, was to replicate current ecological changes in tropical reef habitats such as reduced pH, elevated nutrients and increased temperatures to determine their effects on viral populations within the coral. The experiments found highly-abundant levels of a herpes-like viruses in stressed Porites compressa corals. Interestingly, the viral sequences were rarely detected in un-stressed, healthy samples.
Although corals are the main builders of tropical reef systems, little is known about their microbiology. As a result, studies on coral disease have been limited to bacteria and fungus that can be easily cultivated. The depth of coverage and absence of cloning steps make 454 Sequencing ideally suited for unbiased metagenomic surveys of highly diverse samples.
In this study, the Genome Sequencer system enabled the identification of over 15,000 coral associated viral sequences and, among those, herpes viruses were the most common.
“The sheer number of available sequences has allowed me to demonstrate that the similarities have broad and deep coverage across the a-herpesvirus genomes and are not due to bias in the database,” states lead author Rebecca Vega Thurber, Assistant Professor of Biology at Florida International University and former member of Forest Rohwer’s lab in San Diego. “Most importantly, these sequences have allowed us to use alternative measures to test and confirm the hypothesis that stress causes production of these herpes-like viruses.”
“Dr. Rohwer’s study of how climate change is impacting tropical coral reefs by inducing changes in the viral community of that ecosystem is extremely interesting and important research,” said Christopher McLeod, President and CEO of 454 Life Sciences. “We are excited to see that metagenomic analyses using long reads and high-throughput of our 454 Sequencing system are enabling discoveries in completely new fields of biology, such as coral virology, that were simply not possible only a few years ago.”
While further studies are needed to understand how these viruses affect coral physiology, it is clear that environmental stressors not only result in coral symbiont loss and bacterial and fungal diseases but also in increased herpes-like virus production that together may explain the devastating death of coral reefs as ocean temperatures rise and pollution increases.