Bio-Rad Laboratories, Inc. has announced the availability of a technical paper (bulletin 5420A) that compares the performance of Bio-Rad’s Experion™ system to other methods of RNA quantitation. The bulletin examines the linear range, reproducibility, and accuracy of quantitation by the methods described in the paper and the compatibility of these methods with several common contaminants of RNA preparations. The six-page bulletin describes the methods, results and conclusions in detail, which is supported by numerous graphs, tables and figures.
The Bio-Rad Experion automated electrophoresis system is a valuable tool for the assessment of RNA concentration, integrity, and purity. It can be favorably compared to other more conventional RNA quantitation methods with regards to assay linearity, reproducibility, and accuracy. Additionally, the Experion analysis, in contrast to UV spectroscopy, is relatively unaffected by reagents common to RNA preparation, such as phenol and monophasic phenol-guanidinium isothiocyanate solutions. The additional microfluidic separation affords the unique ability to visually evaluate the RNA sample for degradation and for the presence of copurifying nucleic acids, such as gDNA and tRNA.