A Life Sciences Renaissance: 100th Peer-Reviewed Publication Enabled by 454 Sequencing™
News Nov 07, 2007
454 Life Sciences has announced the publication of over 100 peer-reviewed studies using the Company's Genome Sequencer System.
The studies span a diverse group of DNA sequencing applications, including de novo sequencing and re-sequencing of whole genomes, metagenomics, RNA analysis, and targeted sequencing of DNA regions of interest.
The Genome Sequencer System, powered by 454 Sequencing, enables researchers to tackle research, publish faster, and access diverse applications.
According to 454 Sequencing, its feature set of long reads, accuracy, and ultra-high throughput has enabled research, as evidenced by the large number of studies which have appeared in top tier journals, including 12 papers in Nature, 11 papers in Science, 10 papers in Nucleic Acids Research, 9 papers in Genome Research, 8 papers in PNAS, and 4 papers in Cell.
"454 Sequencing was the innovation we needed to make metagenomics a routine method for accessing viral and microbial environmental communities." said Forest Rhower, PhD., Associate Professor of Biology at San Diego State University.
"The long reads and high throughput of the GS FLX allow my lab to sequence metagenomes, from coral reefs to the human lungs, and determine the types of organisms that inhabit these environments and their metabolic potential," Rhower added.
The first generation Genome Sequencer System, the GS 20, was launched two years ago. To perform, analyze, write and submit a paper for a peer-reviewed publication is usually a year-long process. The speed with which researchers using the Genome Sequencer system have published their discoveries demonstrates the high-quality and easy analysis of 454 Sequencing results.
The 100th study, entitled "A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexing," appeared October 11th in the journal Nucleic Acids Research.
The Stanford based study, led by 2006 Nobel Laureate Andrew Fire, explored how an individual DNA sample could be uniquely "barcoded" with the addition of a known short nucleotide sequence to the ends of all DNA molecules in that sample. Barcoded samples can be pooled together to improve the flexibility of experimental design and the ability to multiplex more samples on a single sequencing run. This study and the others can be found, sorted by application, on the company's website.
The studies published to date come from the fields of cancer research, infectious diseases research, drug discovery, marine biology, anthropology, paleontology, and many more. The 100 studies are complemented by numerous review articles that explore the current uses and future potential of 454 Sequencing.