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ABI Makes Primer Sequences Available Through NCBI Website

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Applied Biosystems has announced that it has contributed 400,000 primer-pair designs, that cover nearly 16,000 human genes, to the new probe database developed by the National Center for Biotechnology Information (NCBI) in the US.

These primer sequences, developed for the Applied Biosystems VariantSEQr™ Resequencing System, will allow DNA sequence variants associated with disease and other phenotypes to be detected easily by resequencing.

The freely available Applied Biosystems primer designs include full gene coverage, with primers for all exons (coding and non-coding), intron/exon splice junctions and 5' regulatory regions.

Each gene is annotated to show regions where there is less than 100 per cent coverage and each primer-pair's performance is given a predicted confidence value.