Agilent Launches One-Step Setup RT-PCR Kit for Cloning, Gene Expression and Quantification
News Jan 08, 2010
Agilent Technologies Inc. has announced availability of the AffinityScript One-Step RT-PCR Kit. The launch of the AffinityScript One-Step RT-PCR Kit represents a re-entry into the one-step RT-PCR market for Agilent’s Stratagene Products Division with a solution for researchers performing end-point RT-PCR.
The AffinityScript One-Step RT-PCR Kit is a high-performance system for high-throughput RT-PCR in a one-step reaction. The kit is designed for easy one-step set-up for RT-PCR, and the simple format eliminates the potential for contamination when preparing multiple or high-throughput reactions. The kit targets researchers performing end-point RT-PCR reactions and provides high product yields and fidelity for applications such as cloning, gene detection, and quantification.
“This is the only RT-PCR kit on the market with fusion enzyme-based technology, enabling faster extension times, substantially reduced time-to-results, and increased sample throughput without compromising amplicon yields and template input sensitivity,” said Nick Price, Ph.D., product manager at Agilent Technologies.
The kit consists of AffinityScript RT and Herculase II Fusion DNA polymerase enabling multi-temperature RT, with higher activity than the leading competitor’s kits at all temperatures, and a robust amplification step to generate higher yields of cDNA with amplicons ranging from 500 bp to 9.6 kb.
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.
Researchers published today a detailed description of the complete genome of bread wheat, the world's most widely-cultivated crop. This work will pave the way for the production of wheat varieties better adapted to climate challenges, with higher yields, enhanced nutritional quality and improved sustainability.