Analysis of RNA Polymerase II Mutants
News Oct 22, 2009
In a recent research study, M. Heidemann used Roche´s xCELLigence RTCA SP Instrument for in vitro testing of cells (SIX: RO, ROG; OTCQX: RHHBY) to test entirely synthetic CTDs. According to his findings, the new xCELLigence System technology allows for the first time the monitoring of cell proliferation and cell death throughout the entire experiment, constantly recording cellular effects during cell plating, transfection, and compound treatment. This non-invasive and continuous monitoring of cells provides a far more detailed picture into the whole experimental process, revealing both specific and off-target effects that may have been missed using conventional endpoint assays.
The precise regulation of gene expression is a very important feature of how organisms respond to environmental changes and regulate cell proliferation, development, and even programmed cell death. Gene expression starts with the transcription of genomic DNA into messenger RNA, the template for protein synthesis during ribosomal translation. RNA polymerase Pol II is one out of three different RNA polymerases used by eukaryotic cells to produce the main categories of cellular RNA. In contrast to Pol I and Pol III, Pol II transcripts are translated into proteins. Pol II is also modified by enzymes influencing different stages of transcription, processing of premature mRNAs in a promoter-dependent manner. These modifications occur in a domain at the carboxy-terminal part of the largest subunit of Pol II, which has a very unique structure.
Previous work by the International Multiple Sclerosis Genetics Consortium (IMSGC) has identified 233 genetic risk variants. However, these only account for about 20% of overall disease risk, with the remaining genetic culprits proving elusive. A new study has tracked down four of these hard-to-find genes.READ MORE