Analysis of RNA Polymerase II Mutants using Roche’s xCELLigence System
News Oct 23, 2009
The C-terminal domain (CTD) of RNA Polymerase II consists of multiple repeats of an evolutionary conserved motif of seven amino acids, forming a repetitive structure of 52 repeats in humans and 26 repeats in yeast. Twenty years after the first description of the RNA polymerase II C-terminal domain, its function is still unclear.
In a recent research study, M. Heidemann used Roche’s xCELLigence RTCA SP Instrument for in vitro testing of cells to test entirely synthetic CTDs.
According to his findings, the new xCELLigence System technology allows for the first time the monitoring of cell proliferation and cell death throughout the entire experiment, constantly recording cellular effects during cell plating, transfection, and compound treatment. This non-invasive and continuous monitoring of cells provides a far more detailed picture into the whole experimental process, revealing both specific and off-target effects that may have been missed using conventional endpoint assays.
The precise regulation of gene expression is a very important feature of how organisms respond to environmental changes and regulate cell proliferation, development, and even programmed cell death. Gene expression starts with the transcription of genomic DNA into messenger RNA, the template for protein synthesis during ribosomal translation. RNA polymerase Pol II is one out of three different RNA polymerases used by eukaryotic cells to produce the main categories of cellular RNA.
In contrast to Pol I and Pol III, Pol II transcripts are translated into proteins. Pol II is also modified by enzymes influencing different stages of transcription, processing of premature mRNAs in a promoter-dependent manner. These modifications occur in a domain at the carboxy-terminal part of the largest subunit of Pol II, which has a very unique structure.
APC Protein Deletion Disrupts Cell Signalling and Could Cause AutismNews
Researchers show deletion of the protein APC in progenitor cells leads to massive disruption of brain development and a signaling cascade previously linked to genes associated with autism.READ MORE
The Ancient Behaviour of Sleep, Conserved Throughout EvolutionNews
The finding that jellyfish sleep implies that sleep is an ancient behavior, largely untouched by millennia of evolution.READ MORE
Gene Immunotherapy Approach Prevents and Reverses Symptoms of Multiple SclerosisNews
Researchers used a viral vector to deliver a gene encoding a myelin sheath protein to the liver, thereby inducing robust and durable immune tolerance in mice by preventing T cells from attacking the myelin sheath.READ MORE