Automating DNA Isolation from Cereal Crops with Tecan’s Magnetic Bead Technology
News Jul 04, 2008
Molecular biologists at the Research Centre for Agriculture and Forestry Laimburg in Northern Italy, have developed a semi-automated procedure for extracting and isolating high quality DNA from cereal crops, using the Te-MagS™ magnetic separation module from Tecan.
The researchers are performing large-scale microsatellite marker analysis to genetically characterize local cereal landraces, in order to establish and maintain a germplasm collection for these crops.
“Isolation of DNA from plant tissue represents a crucial step for the quality and outcome of subsequent downstream applications, such as PCR, sequencing or genotyping, but this step can also be a considerable bottleneck for extensive plant genotyping studies,” explained Dr Sanja Baric, head of the Molecular Biology Section at the research centre.
“The semi-automated magnetic bead-based separation procedure that we have established with the Te-MagS has at least doubled our throughput and more than halved the manual labor time compared to conventional cetyl-trimethylammonium bromide (CTAB)-based protocols.”
Dr Baric’s set-up includes a Tecan Freedom EVO® 100 liquid handling workstation with an integrated Te-MagS module for fully automated DNA isolation from manually prepared plant lysates. The final DNA elutes from 48 samples can be prepared within 90 minutes, and are of sufficient quality to give amplification of many different microsatellite loci.
Researchers Awarded $28M for Illuminating Druggable Genome NIH GrantsNews
Researchers receive grants as part of the NIH program focused on experimental and informatics approaches to characterize understudied proteins from three gene families: ion channels, G protein-coupled receptors (GPCRs), and protein kinases.READ MORE
No Country for Old GenesNews
Our modern world is radically different from the one we evolved in, and that creates a mismatch between the environment our genes were evolved to face, and the world those genes now encounter. A new review looks at how certain genes that benefited humans in our genetic past now predispose us to disease in old age.READ MORE
CRISPR Editing Stops HIV Virus in Infected CellsNews
Human immunodeficiency virus-1 (HIV-1) infection is a chronic disease affecting more than 35 million people worldwide. The infection can be controlled by antiretroviral therapy (ART), but there is still no complete cure. Now, a new study targeting the regulatory genes of the virus using CRISPR/Cas9 has helped block the production of the virus by infected cells.READ MORE