Automating DNA Isolation from Cereal Crops with Tecan’s Magnetic Bead Technology
News Jul 04, 2008
Molecular biologists at the Research Centre for Agriculture and Forestry Laimburg in Northern Italy, have developed a semi-automated procedure for extracting and isolating high quality DNA from cereal crops, using the Te-MagS™ magnetic separation module from Tecan.
The researchers are performing large-scale microsatellite marker analysis to genetically characterize local cereal landraces, in order to establish and maintain a germplasm collection for these crops.
“Isolation of DNA from plant tissue represents a crucial step for the quality and outcome of subsequent downstream applications, such as PCR, sequencing or genotyping, but this step can also be a considerable bottleneck for extensive plant genotyping studies,” explained Dr Sanja Baric, head of the Molecular Biology Section at the research centre.
“The semi-automated magnetic bead-based separation procedure that we have established with the Te-MagS has at least doubled our throughput and more than halved the manual labor time compared to conventional cetyl-trimethylammonium bromide (CTAB)-based protocols.”
Dr Baric’s set-up includes a Tecan Freedom EVO® 100 liquid handling workstation with an integrated Te-MagS module for fully automated DNA isolation from manually prepared plant lysates. The final DNA elutes from 48 samples can be prepared within 90 minutes, and are of sufficient quality to give amplification of many different microsatellite loci.
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.
Researchers published today a detailed description of the complete genome of bread wheat, the world's most widely-cultivated crop. This work will pave the way for the production of wheat varieties better adapted to climate challenges, with higher yields, enhanced nutritional quality and improved sustainability.