Bioo Scientific has announced that the United States Patent and Trademark Office (USPTO) has issued U.S. patent US9255291 entitled Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing. The granted claims describe a method for improving small RNA NGS library prep by reducing bias, formation of adapter dimers and other unwanted side products.
The industry-leading NEXTflex™ Small RNA-Seq Kit v3 not only utilizes this patent but also incorporates patent pending randomized adapter and molecular counting technology to reduce bias introduced during the ligation step of library prep and improve accuracy. The ligation step of small RNA library prep is ordinarily a source of severe bias. The randomized adapter strategy incorporated into the NEXTflex Small RNA-Seq Kit v3 allows small RNAs of any sequence to “find” their respective optimal adapters, resulting in small RNA libraries with a dramatic reduction in bias.
This kit also allows the typical gel size selection step to be replaced with a bead purification step with input of 200 ng -2 µg total RNA, or facilitates traditional small RNA library prep from input as low as 5 ng of total RNA. According to Dr. Masoud Toloue, Bioo Scientific’s Vice President of Genomic Research, “The addition of this patent to Bioo Scientific’s IP portfolio demonstrates our effort to reduce biases associated with library preparation and improve sequencing accuracy.”