Comparison of Real-Time Quantitative PCR and Culture for the Diagnosis of Emerging Rickettsioses
News Mar 20, 2012
Diagnosis of Rickettsia infection would benefit by use of the more rapid and sensitive method of quantitative realtime PCR than the time-intensive and less sensitive method of culturing Rickettsia species from skin biopsies. We evaluated culture sensitivity compared to PCR according to sampling delay and previous antibiotic treatment. We found that skin biopsies can be positive even when molecular tests were negative, and a negative result using molecular assays did not exclude the diagnosis of Rickettsia spp. infection. Rickettsia africae was the most common species in skin biopsies and R. slovaca was most common in ticks. We found a positive correlation between the number of bacteria copies and the isolation success in skin biopsies and ticks. The probability of isolating Rickettsia spp. was higher in untreated patients and in patients from our hometown. To increase the sensitivity of culture, skin biopsies should be sampled before treatment early in the course of the disease and should be inoculated as soon as possible.
This article is published online in PLoS ONE and is free to access.
Breath Test Could Simplify Drug and Disease TestingNews
Testing for drug use and disease in humans could soon be much simpler, thanks to new Swedish research.Scientists have demonstrated how collecting and analysing externally-produced compounds in the lining fluid of the airways allows for non-invasive testing and monitoring.The researchers used liquid chromatography-mass spectrometry to analyse the collected samples, as well as investigating the impact of different breathing patterns.READ MORE
Kidney Disease Diagnosis May Benefit from DNA SequencingNews
DNA sequencing yielded clinically meaningful insight into the cause, and treatment, of many types of kidney disease.READ MORE