Fluidigm and the LICR Join Forces to Measure Expression of 900 Genes in 96 Samples in One Week
News Jun 13, 2008
Fluidigm announced that the San Paulo Branch of the international Ludwig Institute for Cancer Research (LICR) used the company’s BioMark™ System to conduct a large scale analysis characterizing the whole human “surfaceome” (the set of genes encoding cell surface proteins) in what was for them record time. The experiment compressed months of work for the LICR team into just one week at Fluidigm’s South San Francisco lab.
The LICR team used tumor needle biopsies of colon, lung, prostate and breast samples (as well as control samples) to conduct its experiments. Using Fluidigm’s new 96.96 dynamic arrays, Dr. da Cunha was able to process 55,000 data points in a day with just four hours of hands-on labor.
Gajus Worthington, Fluidigm’s President and CEO, noted that the Ludwig Institute of Cancer Research’s work currently uses the company’s 48.48 dynamic arrays.
“But, with our newly introduced 96.96 dynamic arrays, all this gene expression work could now be completed faster, or four times as many samples can be finished in the same week at even lower cost.”
With the availability of the human genome sequence, as well as a large amount of data on expressed genes in cancer, the LICR is conducting several studies attempting to characterize genes differentially expressed in cancer.
Sandro J. de Souza, Ph.D., of the Ludwig Institute said, “We have performed a large-scale analysis aimed to characterize the whole human “surfaceome,” integrated gene expression data into a “surfaceome” knowledge base, identified gene candidates to be differentially expressed in glioblastoma and colon tumors and want to validate some of these candidates using a large-scale qPCR strategy. Fluidigm’s 96.96 dynamic arrays will play a critical role in the qPCR phase of our research.”
Fluidigm’s BioMark™ 96.96 Dynamic Array is capable of performing 9,216 simultaneous real-time PCR experiments running gold-standard TaqMan® assays in nanoliter quantities.
When comparing Fluidigm’s new 96.96 Dynamic Arrays to industry-standard 384-well systems using 10 uL reaction volumes, the 96.96 provides significant improvements in productivity and efficiency, Fluidigm said.
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.
Researchers published today a detailed description of the complete genome of bread wheat, the world's most widely-cultivated crop. This work will pave the way for the production of wheat varieties better adapted to climate challenges, with higher yields, enhanced nutritional quality and improved sustainability.