Helicos BioSciences Announces Genome Sequencing Using True Single Molecules
News Dec 20, 2005
Helicos BioSciences has announced that it has sequenced the M13 genome, by utilizing its non-amplified True Single Molecule Sequencing (tSMS™) technology in conjunction with its SimplePrep™ genome preparation technique.
Sequencing of M13 was conducted on the entire genome, including so-called homopolymeric sequences.
"This marks an unprecedented achievement," said Stanley N. Lapidus, President and CEO of Helicos BioSciences.
"Helicos is the only organization to have successfully sequenced a genome using tSMS™ technology - an accomplishment we've worked hard to achieve."
He added, "The simplicity of tSMS™ in working directly from genomic DNA and eliminating amplification, provides researchers with unparalleled advantages, potentially enabling them to represent the entire human genome on a single glass substrate - and to sequence it in a day, and not years."
Using EBX reagents, researchers have converted the C-terminal carboxylic acid of peptides into a carbon-carbon triple bond - an alkyne (in chemical jargon a "decarboxylative alkynylation"). The alkyne moiety is a very valuable functional group that can be used to further modify the peptides.READ MORE