Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has developed an extensive quantitative real-time polymerase chain reaction (qPCR) user guide, which is available to download for free from www.idtdna.com. Providing user guidance on the entire qPCR process - from RNA isolation to data analysis - the manual covers the basics of experimental set-up, performance and analysis.
Specific information on 5’ nuclease assays, including re-suspensions and qPCR protocols are also supplied, as well as a troubleshooting section which discusses commonly encountered issues. The entire document is written in compliance with MIQE guidelines: minimum information for publication of quantitative real-time PCR1.
IDT offers a broad range of PrimeTime qPCR assay products including customized primers and probes, in addition to the free online design suite - SciTools