We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Image Analyser used at Major US Pain Research Centre Improves Accuracy of Detecting Proteins Associated with Spinal Cord Injury
News

Image Analyser used at Major US Pain Research Centre Improves Accuracy of Detecting Proteins Associated with Spinal Cord Injury

Image Analyser used at Major US Pain Research Centre Improves Accuracy of Detecting Proteins Associated with Spinal Cord Injury
News

Image Analyser used at Major US Pain Research Centre Improves Accuracy of Detecting Proteins Associated with Spinal Cord Injury

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Image Analyser used at Major US Pain Research Centre Improves Accuracy of Detecting Proteins Associated with Spinal Cord Injury"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Syngene has announced that a specialist US medical institute, the Dartmouth-Hitchcock Medical Centre (DHMC), is significantly improving detection of proteins associated with spinal cord injuries, using a G:BOX Chemi HR16, Syngene’s innovative fluorescence and chemiluminescence image analyser.

Scientists in the Department of Anesthesiology at DHMC are using a G:BOX Chemi HR16, supplied by New England BioGroup, LLC (Syngene’s exclusive representative in New England) to image Western blots of HRP labelled proteins that are involved in inflammatory processes, (p-ERK, total-ERK, MKP1, NOS2, IL-1 and TNFa) isolated from neonatal cortical microglial cells treated with new drugs, post peripheral nerve injury. By identifying a protein’s presence, DHMC researchers hope to determine which drugs most effectively treat the pain caused by peripheral nerve injury.

Dr Edgar Alfonso Romero-Sandoval, Instructor of Anesthesiology at the DHMC explained: “Pain following peripheral nerve injury often responds poorly to available therapies, which is why we are looking at new drugs to treat it. To do this we use expensive rat primary cell cultures that yield little protein and were using a laser scanner to image our Westerns but could not detect the microgram amounts of proteins we had, as there was so much background.”

Dr Romero-Sandoval added: “We switched to a G:BOX Chemi HR16 because it allows us to adjust exposure conditions, (something we couldn’t do with our laser scanner) to detect these tiny protein amounts. Using a G:BOX Chemi HR16 has improved the sensitivity of imaging our Western blots so much, we have been able to reduce the detecting antibody concentration by 2-4 fold and significantly lowered reagent costs. Additionally, with a G:BOX we can strip our membranes several times and are still able to detect different proteins using the same blot derived from the same experiment and cell culture.”

Paula Maia, Vice President of Sales, Syngene US stated: "We are pleased our G:BOX Chemi HR16 system is being utilised in such important pre-clinical studies. The research at the DHMC demonstrates to any lab trying to maximise accuracy of protein detection, while minimising costs that choosing a G:BOX Chemi HR16 system will be a decision without compromise.”

Advertisement