Invitrogen and Greiner Bio-One Team to Increase HPV Test Reliability
News Jun 26, 2007
The agreement covers the rights for the use of dUTP (deoxyuridine triphosphate) in PCR-based methods to prevent sample contamination by amplification products in aerosols.
“The overall benefit of using the Invitrogen dUTP during the PCR is that false-positive results are reliably prevented at the beginning of the procedure, while the genuine sample DNA remains intact,” said Heinz Schmid, Greiner Bio-One managing director.
In order to avoid false-positives for PCR-based methods, Greiner Bio-One is incorporating into two of its DNA-arrays a procedure patented by Invitrogen for preventing PCR carryover contamination.
During the PCR, dUTP is used instead of dTTP (deoxythymidine triphosphate). Any potential carried-over contaminating PCR-products can then be selectively degraded before starting a new reaction by the addition of the enzyme Uracil-DNA-glycosylase.
“Greiner Bio-One’s HPV test will now deliver more reliable results for women who may need to be treated for HPV, which is the leading cause of cervical cancer,” said Amy Butler, Invitrogen vice president of gene expression profiling. "Licensing patented technologies like dUTP is allowing Invitrogen to assist diagnostic companies around the world to improve the sensitivity and specificity of their offerings."
Beginning in June, Greiner Bio-One will deliver the product kits for PapilloCheck® with dUTP in the MasterMix, and it will be included in the product kits for CytoCheck® this fall.
Mechanism Controlling Multiple Sclerosis Risk IdentifiedNews
Researchers at Karolinska Institutet have now discovered a new mechanism of a major risk gene for multiple sclerosis (MS) that triggers disease through so-called epigenetic regulation. They also found a protective genetic variant that reduces the risk for MS through the same mechanism.
Antarctic Worm and Machine Learning Help Identify Cerebral Palsy EarlierNews
A research team has released a study in the peer-reviewed journal BMC Bioinformatics showing that DNA methylation patterns in circulating blood cells can be used to help identify spastic cerebral palsy (CP) patients. The technique which makes use of machine learning, data science and even analysis of Antarctic worms, raises hopes for earlier targeted CP therapies.
Ancient Syphilis Genomes Decoded for First TimeNews
Researchers recovered three genomes of the bacterium Treponema pallidum from skeletal remains from colonial-era Mexico, and were able to distinguish the subspecies that causes syphilis from the subspecies that causes yaws. It was not previously thought possible to recover DNA from this bacterium from ancient samples.