Knockdown Cell Lines for DNA Repair Studies
News Feb 23, 2011
AMSBIO has announced a new range of highly efficient knockdown cell lines covering the Base Excision DNA Repair pathway.
The new range of 20 DNA Repair Knockdown cell lines was developed to enable scientists to study the molecular etiology of tumour genomic instability and to exploit it in oncology research. The AMSBIO DNA Repair Knockdown cell lines are based upon target-specific LN428 glioblastoma shRNA lentivirus transduced cells that are rigorously qualified and mycoplasma free.
DNA repair pathways maintain the integrity of the genome, reducing the onset of cancer, disease and aging phenotypes. The requirement for DNA repair and genome maintenance in response to radiation and genotoxic chemotherapeutics implicates DNA repair proteins as prime targets for improving response to currently available anti-cancer regimens. AMSBIO's DNA repair knockdown cell lines provide essential tools to address these needs.
All AMSBIO DNA Repair Knockdown Cell Line products are evaluated by RT-PCR and Western Blots in addition functional assays are performed were feasible. The knockdown efficiency of the new DNA Repair Knockdown Cell Lines on target genes, as measured by RT-PCR, is 63-98%.
Scientists used a gene editing method called CRISPR/Cas9 to generate mice that faithfully mimic a fatal respiratory disorder in newborn infants that turns their lips and skin blue. The new laboratory model allowed researchers to pinpoint the ailment's cause and develop a potential and desperately needed nanoparticle-based treatment.READ MORE