Increased Understanding of DNA Repair Mechanisms May Lead to New Cancer Therapeutics
Credit: Mayo Clinic
We humans like to think our DNA is well-protected in the nucleus of each cell. But it’s a hard life for the hard-working genetic code.
DNA can be damaged by a range of normal cellular activities, not to mention ultraviolet light and ionizing radiation. The bad news is that damage, such as DNA double-strand breaks, is constantly occurring. But the good news is the human body has so-called damage response proteins to fix it.
Now, thanks to a new publication by Mayo Clinic researchers, scientists know more about these damage response proteins and how they do their job.
“This basic research is aimed at understanding how DNA damage response proteins work,” explains Georges Mer, Ph.D., a Mayo Clinic biochemist. “We hope the knowledge gained from these studies might, in the long term, be beneficial from a therapeutic perspective, notably for cancer treatment.”
Certain types of radiation, such as gamma rays and X-rays, can cause breaks in both strands of DNA. If it’s unfixable, the damage can trigger a cell to die. But Dr. Mer and team examined the way in which these breaks can trigger chemical signals to mobilize the DNA repair proteins.
Within the nucleus of the cell, DNA is wrapped around proteins called histones. A package of eight histones and DNA is called a nucleosome. When a DNA double-strand break occurs, histones are chemically modified at different positions. The authors studied the modification that occurs when a molecule called ubiquitin attaches to a histone in response to the DNA break. They examined the attachment of ubiquitin on the H2A histone at two positions, and, when the call goes out, how the damage response protein attaches to the nucleosome.
“By deriving a detailed 3-D structure of [the damage response protein] RNF169 bound to the ubiquitylated nucleosome we explain how specificity is achieved in ubiquitin recognition,” says Dr. Mer.
Expanded knowledge for future therapy
Many important regulatory interactions in the cell rely on recognition of ubiquitin-modified proteins. Structural studies like this one help define how ubiquitin is selectively recognized, as can be seen in the above image.
In this study, published in Molecular Cell, the authors also report that when RNF169, the DNA damage repair protein, binds to the nucleosome, it prevents another DNA repair protein from associating with the nucleosome. That other repair protein, 53BP1, shuts down an alternate pathway to repair DNA double-strand breaks. The authors note that inhibiting this inhibitor could, in principle, be exploited to reactivate this alternate DNA double-strand break pathway in cases where it is defective as in some forms of breast and ovarian cancer.
Hu, Q., Botuyan, M. V., Cui, G., Zhao, D., & Mer, G. (2017). Mechanisms of Ubiquitin-Nucleosome Recognition and Regulation of 53BP1 Chromatin Recruitment by RNF168/169 and RAD18. Molecular Cell. doi:10.1016/j.molcel.2017.04.009