We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Not a Constant Value: Understanding Tm
News

Not a Constant Value: Understanding Tm

Not a Constant Value: Understanding Tm
News

Not a Constant Value: Understanding Tm

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Not a Constant Value: Understanding Tm"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

In the latest issue of DECODED, the quarterly newsletter form Integrated DNA Technologies, resident expert on nucleic acid thermodynamics, Dr Richard Owczarzy has introduced researchers to a new approach for calculating nucleic acid melting temperature (Tm).

Exploring the impact of environmental conditions on Tm, the article explains how researchers can use these calculations to enhance the performance of their hybridization-based protocols.

Oligonucleotide hybridization forms the basis of many molecular biology techniques, from PCR and NGS through to in situ hybridization and gene knockdown studies, and knowing the exact Tm is vital when designing these experiments.

Dr Owczarzy explains how many researchers simplify their calculations of Tm as a fixed characteristic based on sequence composition.

However, Tm is also dependent on experimental conditions, including oligo concentration, the ionic environment and Mg2+ availability, as well as sequence mismatches and SNPs.

IDT has therefore developed a free online Tm calculator, delivering the most accurate calculations of Tm.

Advertisement