Oncolytics Collaborators to Present Preclinical Research in Multiple Myeloma and Melanoma
News Apr 08, 2014
Oncolytics Biotech Inc. has announced that research collaborators will make two poster presentations at the American Association for Cancer Research 2014 Annual Meeting. The Meeting is being held from April 6th to 9th, 2014 in San Diego, CA.
"The data presented by these collaborators is contributing to our evolving understanding of how REOLYSIN® works, what biomarkers may be predictive of improved patient outcomes, and the potential synergies with other drugs in specific indications," said Dr. Brad Thompson, President and CEO of Oncolytics. "There is ongoing clinical work in both multiple myeloma and metastatic melanoma and these data will help shape future studies in these indications."
The first poster, titled "Reovirus synergy with proteosome inhibitor carfilzomib and Akt inhibitor perifosine overcomes therapy resistance of multiple myeloma: promising preclinical activity," is being presented by Thirukkumaran et al.
The poster presents findings demonstrating that reovirus acts synergistically with carfilzomib and/or perifosine in oncolysis of therapy resistant multiple myeloma cell lines. Synergistic cytotoxicity was observed with reovirus and carfilzomib and perifisone in both reovirus resistant OPM2, KMS-11 or sensitive RPMI8226 cell lines. Reovirus resistant cell lines demonstrated enhanced synergism in comparison to reovirus sensitive RPMI8226.
The second poster, titled "Molecular and immunological correlates of latent versus productive reoviral infection in cancers" is being presented by Mansfield et al. The poster presents findings following analysis of several human multiple myeloma cell lines either resistant or sensitive to reovirus infection and a metastatic melanoma model in mice after reoviral infection.
Myeloma cell lines resistant to reoviral infection showed that the majority of the cancer cells had latent reoviral infection with very weak productive infection (productive/latent reoviral infection 6%). Myeloma cells sensitive to reoviral infection showed many cells with a productive viral infection (productive/latent reoviral infection was 50%). P38 expression was identical in the resistant and sensitive myeloma cell lines; two molecular markers that marked the reoviral sensitive myeloma cell lines were high CUG2 expression and low ammonia production.
In the metastatic melanoma model, latent infection was dominant in mice treated with reovirus alone; productive infection was dominant in mice treated with reovirus and chemotherapy. In both the cell lines and metastatic melanoma model, productive infection was associated with elevated caspase-3 expression. In the metastatic melanoma model, productive infection was associated with increased T cell and natural killer cell infiltration of the tumor.
The posters will be available on the Oncolytics website at http://www.oncolyticsbiotech.com/for-investors/presentations.
A clinical study entitled "Viral Protein Production After Dexamethasone, Wild-Type Reovirus, and Carfilzomib in Treating Patients With Multiple Myeloma" will examine the use of REOLSYIN® in combination with carfilzomib (ClinicalTrials.gov Identifier: NCT02101944) in patients with relapsed or refractory myeloma.