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Polarized Fluorescent Probe Reveals Architectural Dynamics of Living Cells

News   May 14, 2021 | Original story from Tokyo Medical and Dental University

 
Polarized Fluorescent Probe Reveals Architectural Dynamics of Living Cells

A. Live-cell fluorescence polarization imaging of the first cleavage of a starfish embryo expressing POLArIS that specifically binds to F-actin. Vertical and horizontal actin filaments are shown in magenta and green, respectively. FLARE structures made of radially extending actin filaments were observed as cross-patterns of magenta and green. Scale bar, 50μm. B. A starfish embryo in the first cleavage was fixed, stained with fluorescent-dye-conjugated phalloidin (stains F-actin) and anti-tubulin antibody (stains microtubules), and observed with confocal laser scanning microscope. Yellow circles, regions of FLARE structure; Red circles, regions of astral microtubules; green box, mitotic spindle; orange arrows, centrosomes. Scale bar, 100 μm. Credit: Department of Neuroanatomy and Cellular Neurobiology/ TMDU

 
 
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