Purdue University scientists have announced that they have constructed tiny delivery vehicles by using strands of genetic material. The vehicles can carry anticancer therapeutic agents directly to infected cells, offering a potential wealth of treatments for chronic diseases. These so-called nanoparticles, which are assembled from three short pieces of ribonucleic acid, resemble miniature triangles.
The microscopic particles possess both the right size to gain entry into cells and also the right structure to carry other therapeutic strands of RNA inside with them, where they are able to halt viral growth or cancer's progress. The team has already tested the nanoparticles against cancer growth in mice and lab-grown human cells.
"RNA has immense promise as a therapeutic agent against cancer, but until now we have not had an efficient system to bring multiple therapeutic agents directly into specific cancer cells where they can perform different tasks," said research team leader Peixuan Guo, who is a professor of molecular virology at Purdue with joint appointments in Purdue's Cancer Research Center, School of Veterinary Medicine and Weldon School of Biomedical Engineering."Physicians have hoped that nanotechnology might provide a solution to the problem, and it's possible that the application of these tiny triangles could lead to the solution."
Guo's team created their nanoparticles by linking together different kinds of RNA, a task that their previous research has given them ample opportunities to practice. Several years after building a tiny "motor" from several strands of RNA that mimic those in a bacteria-killing virus called phi29, the team learned how to manipulate these stringy molecules into different shapes, including rods, triangles and arrays.
“We speculated at that time that these shapes would be useful purely as physical scaffolding on which more sophisticated nanodevices could be constructed," Guo said. "But RNA, which carries genetic messages within cells, also has many therapeutic functions.” “We realized that if we built different kinds of therapeutic RNA onto the RNA scaffolding and created a single structure, we might be able to respond to several challenges that have confronted the medical field."
RNA molecules come in many variant forms, and the sort that the team mimicked from the phi29 virus – called pRNA – also can be linked to other types of RNA to form longer, hybrid strands with properties the researchers could assign.
"We looked around for RNA strands that would behave in certain ways when they encounter a cancer cell because each of them needs to perform one step of the therapy," Guo explained. "An effective agent against cancer needs to accomplish several tasks. It needs first to recognize the cancer cell and gain access to its interior, and then it needs to destroy it.” “But we'd also like the agent to leave a trail for us, to mark the path the molecule has taken somehow.”“That way, we can pinpoint the location of the cancer and trace the outcome after the treatment."
To accomplish these tasks, the team turned to other forms of RNA that can interfere with the goings-on inside cells. The team sorted through a variety of RNA forms that have shown promise for disease treatment and found three that could perform each of the desired tasks. One example is "small interfering RNA," or siRNA, which deactivates certain genes in cells. The others are RNA aptamers, which bind to cancer cell surface markers, and ribozymes, which can be designed to degrade specific RNA in cancer cells or viruses.
"We linked each of the three therapeutic strands with a piece of pRNA, forming three hybrid strands," Guo said. "Then, using techniques we learned from our earlier work, we were able to combine all three into triangles that are between 25 and 40 nanometers wide.""This is the Goldilocks size for any nanoparticle that is to be used in the body – not too big, not too small."
Particles larger than about 100 nanometers are generally too large to pass through cell membranes into the cell's interior, Guo said, and the body has a hard time retaining particles smaller than 10 nanometers. But the tiny triangles fit, and they worked well enough to interrupt the growth of human breast cancer cells and leukemia model lymphocytes in laboratory experiments.
"One characteristic of cancer cells is that they do not stop growing, which is one reason tumors develop," Guo said. "Once inside, the siRNA essentially instructs the cells to 'stop not stopping.' The nanoparticles had done their work on the breast cancer cell cultures within a few days."
A group of mice that were in the process of developing cancer were tested with the nanoparticles, and they did not develop the disease. A second group that was tested with mutated inactive RNA all developed tumors.
"The results are very promising, but we still have several hurdles to jump before we can test this therapy on people," Guo said. "First and foremost, we must ensure that it is as safe as we think it is. Some RNA can be toxic to noncancerous cells as well, and though our nanoparticles appear to go straight to the cancer cells where we want them to go, we have to be sure they do not go anywhere else before we can inject them into a living person."
"Many studies have shown that therapeutic forms of RNA, such as siRNA or ribozymes, could be put together to kill cancer, but the main obstacle has been finding the delivery method that can bring them to specific cells simultaneously," Guo said. "Nanotechnology is beginning to pay off here in that it may have provided us with a solution to the problem. We hope to enhance the work we have done so far and refine it for human trials."