Redefining High Risk Patients With Stage II Colon Cancer
News Sep 12, 2014
Exiqon A/S has announced the publication of data from a population based study of 554 patients with stage II colon cancer, using the company’s proprietary miRCURY LNA™ Universal RT microRNA PCR platform to validate the prognostic value of microRNA-21 based on tissue sections from formalin fixed paraffin embedded tumour blocks (FFPE samples). End-points were overall survival (OS) and recurrence free cancer specific survival (RF-CSS).
The study results support microRNA-21 as an independent prognostic biomarker for recurrence free cancer specific survival in a population based cohort of patients with stage II colon cancer, which received surgical intervention. Assessment of microRNA-21 levels was based on qPCR analysis of microRNA-21 in standard FFPE samples, and high microRNA-21 expression was associated with an unfavourable RF-CSS. The cross-validated area under the curve (AUC) for 1- and 5-year RF-CSS were 0.67 and 0.68, respectively. The results appeared in British Journal of Cancer
This study confirms results from a previous study published in British Journal of Cancer in 2012, of the same cohort of patients using the company’s proprietary LNA™ based in situ hybridization to analyze microRNA-21 levels. The earlier study showed that patients expressing high levels of miR-21 had significantly inferior recurrence-free cancer-specific survival, suggesting that analyses of microRNA-21 should be considered as a potential adjunct in the selection of high risk stage II colon cancer patients.
The new study tested the prognostic value of a qPCR based assessments of microRNA-21 levels in standard FFPE stage II tumour samples in combination with a traditional panel of prognostic biomarkers to create an individual risk index in patients with stage II colon cancer. Individual biomarkers might not be powerful enough to identify clinically relevant risk groups in a cohort of patients where the majority has already been cured by the surgical intervention and has relatively good prognosis. Hence, the aim of the index approach was to strengthen existing markers by combining these traditional biomarkers with the molecular based biomarker microRNA-21 to provide for a clinically more robust stratification.
The study result suggests that introducing a risk index including microRNA-21 for stratifying patients with stage II colon cancer may lead to the identification of a considerably smaller group of high risk patients (23% of the patients at high risk) than the traditional risk parameters (77% of the patients), holding promise to spare a large fraction of patients from needless and harmful adjuvant chemotherapy. The proposed risk index classifies patients correctly (high or low risk) with a probability of approximately 70%. The cross-validated AUC for the recurrence free cancer specific survival (RF-CSS) index at 1- and 5-year was 0.714 and 0.667, respectively.
The identification of high risk patients is a first step in a possible stratification of patients for adjuvant therapy. The current study does not address whether high risk patients with elevated microRNA-21 expression also will benefit from adjuvant therapy. The possible predictive value of microRNA-21 in identifying high-risk patients who will respond to adjuvant chemotherapy will require further clarification in a clinical context.
With the publication of the current microRNA-21 study, it is clear that microRNA-21 possesses independent prognostic value which in combination with more traditional biomarkers can improve identification of high risk patients. This is a step forward towards better treatment decisions of stage II colon cancer patients who may benefit from being identified as eligible to chemotherapy. Exiqon will proceed with launch of the microRNA-21 test as a RUO (Research Use Only) kit to facilitate further testing and eventually clinical incorporation of microRNA-21 along with other biomarkers to create colorectal cancer recurrence tests. The launch is planned for later this year.
New Microscope Captures Detailed 3-D Movies of Cells Deep Within Living SystemsNews
Merging lattice light sheet microscopy with adaptive optics reveals the most detailed picture yet of subcellular dynamics in multicellular organisms.READ MORE
"Genetic Jenga" Helps Understand How Our Genes Control Our CellsNews
To fully understand how our cells work, we can't focus on just one gene, but must instead look at combinations of genes. Researchers have published a study which knocked out multiple genes, like removing bricks from a Jenga tower, to better understand how they work together.READ MORE
Southeast Asian Nomads Have Evolved to Hunt Fish UnderwaterNews
The human spleen can release oxygenated blood cells to allow more time underwater without breathing. A new study has identified that the "Sea Nomad" Bajau people of Indonesia have evolved larger spleens that would allow them to remain underwater for longer.READ MORE
Comments | 0 ADD COMMENT
World Congress on Advanced Biomaterials and Tissue Engineering
Jun 20 - Jun 21, 2018
29th International Conference on Public Mental Health and Neuroscience
Jul 16 - Jul 18, 2018
International Conference on Epigenetics and Epitranscriptomics
Sep 17 - Sep 18, 2018