Roche’s xCELLigence System for Determination of Optimal Time Points for Gene and Protein Expression Analysis
News Aug 14, 2009
In a recent study, the utility of the Roche xCELLigence System was explored for assessing RNAi-mediated knockdown of gene function. Researchers focused on Eg5, a kinesin involved in the mitosis pathway. Their results provide evidence that real-time cell monitoring using the xCELLigence System is ideal for identifying the optimal time point for further molecular and biochemical analyses of cellular events. The study showed that transfection of siRNA targeted to Eg5 produces a transient cell phenotype, with maximum expression at 27 hours post-transfection.
The real-time online aspect of the xCELLigence System has made it possible to directly link phenotypic cellular events to the molecular and biochemical changes that occur after transfection with siRNA. Conventional analysis of the data at time points before or after 27 hours post-transfection would have missed this optimal window of opportunity for carrying out relevant gene and protein expression assays. Furthermore, the real-time data stream of the xCELLigence System provides a continuous, label-free measure of cell culture quality control for transfection, simultaneously identifying potential off-target effects of the siRNA.
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.
Researchers published today a detailed description of the complete genome of bread wheat, the world's most widely-cultivated crop. This work will pave the way for the production of wheat varieties better adapted to climate challenges, with higher yields, enhanced nutritional quality and improved sustainability.