Sage-N Sorcerer™ Integrates ISB's Open Source Proteomics Tools
News Nov 04, 2005
Sage-N Research, Inc. and the Institute for Systems Biology (ISB) have announced the integration of the ISB Trans-Proteomic Pipeline (TPP) analysis platform into Sage-N Research, Inc.'s Sorcerer appliance.
With this integration research laboratories and core facilities can replicate the power and capability of ISB's high-throughput proteomics analysis tools without requiring extensive information technology (IT) support or costly cluster computers.
The Sorcerer appliance can be plugged into any network to deliver high resolution, high throughput data analysis through a web interface.
With the integration of TPP, a unified web-based tool suite can effectively identify, characterize, and quantify proteins in complex mixtures using any vendor's LC-MS/MS data.
As LC-MS/MS technology continues to become an increasingly powerful and broadly used tool to identify and quantify proteins in complex samples, the proteomics research community faces two key challenges related to the analysis of the large data sets generated by such studies.
First, proteomics laboratories need to build complex IT infrastructures with sufficient computing power to process the collected data, and second, the results obtained from sequence database search engines need to be statistically validated to determine the error rate of the search results.
According to Dr. Ruedi Aebersold, co-founder of the Institute for Systems Biology and principal investigator for the TPP project, “Proteomics research groups and the proteomics community as a whole will greatly benefit from easily accessible and high-throughput systems that analyze LC-MS/MS proteomic data with transparent algorithms and that provide an estimate of the error of the obtained data.”
“The throughput and ease-of-use and ease-of-maintenance of the Sorcerer system with Trans-Proteomic Pipeline support will help to address these needs.”
Sage-N Research claims that, using patent-pending Pattern Match Accelerator™ hardware and systems technology, its Sorcerer appliance offer processing throughput for protein identification that otherwise might require hundreds of compute nodes.
The Sorcerer appliance currently supports the SEQUEST® algorithm, with additional scoring modules being planned.
The Trans-Proteomic Pipeline from the Institute for Systems Biology is an Open Source analysis platform for unified storage and analysis of tandem mass spectrometry data based on open XML file formats.
Using EBX reagents, researchers have converted the C-terminal carboxylic acid of peptides into a carbon-carbon triple bond - an alkyne (in chemical jargon a "decarboxylative alkynylation"). The alkyne moiety is a very valuable functional group that can be used to further modify the peptides.READ MORE