Sigma-Aldrich Expands shRNA Libraries of the RNAi Consortium
News Jan 20, 2006
Sigma-Aldrich has announced the release of the MISSION™ TRC shRNA human and mouse libraries for RNA interference-based research.
The collection now carries approximately 65,000 shRNA vector constructs that target 9,500 human genes and 3,400 mouse genes.
These libraries include extensive coverage of the druggable genome and other highly studied targets.
Each clone is available from Sigma-Aldrich in bacterial glycerol stock, ready-to-transfect purified plasmid DNA, and lentiviral particles for direct transduction.
The lentiviral-based shRNA libraries are designed to provide researchers with tools for studying gene function and disease in standard cell lines and recognized difficult cell types, such as primary, non- dividing, growth-arrested or terminally differentiated cells.
Such cell lines are typically not amenable to standard transfection or genomic integration by other viral delivery systems, but they may be transduced efficiently with lentiviral particles.
Harvard University and The Broad Institute of the Massachusetts Institute of Technology and Design are leading the design and development of the TRC libraries, which will be expanded on a quarterly basis to include new gene targets.
Eventually, the libraries will comprise a comprehensive collection of 150,000 pre-cloned lentiviral-based shRNA vector constructs targeting 15,000 human genes (MISSION TRC-Hs1.0) and 15,000 mouse genes (MISSION TRC-Mm1.0).
Bioethics Council Rules Heritable Genome Editing "Ethically Acceptable" In Certain CircumstancesNews
A leading UK bioethics advisory body has weighed in on the debate around human genetic modification, concluding that heritable genome editing – modifying the DNA of an egg, sperm or embryo with changes that will be passed on to future generations – could be ‘morally permissible’ in humans, provided key ethical tests are met.
Hay Fever Risk Genes Overlap with Autoimmune DiseaseNews
In a large international study involving almost 900,000 participants, researchers from the University of Copenhagen and COPSAC have found new risk genes for hay fever. It is the largest genetic study so far on this type of allergy, which affects millions of people around the world.READ MORE
Hidden Signals in RNAs Regulate Protein SynthesisNews
Scientists have long known that RNA encodes instructions to make proteins. In a new study published in Nature, scientists describe how the protein-making machinery identifies alternative initiation sites from which to start protein synthesis.READ MORE