Small RNA Barcoding for Sample Multiplexing
News Jan 25, 2010
They significantly increase scale and throughput while reducing costs by allowing the user to pool multiple library preparations into a single sequencing reaction. The AIR Barcoded Adapters enable the user to detect rare sample events amongst hundreds of samples, across several time points and even multiple genomes. Predesigned AIR Barcoded Adapters, are available in sets of 10, 20, or 30 and were designed based on uniform melting temperature (Tm) and sequences that have unique color space. Once attached to a sample, they may be pooled together and sequenced in a single flow cell run. Analysis post run allows the user to identify the sequence barcode back to specific samples.
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.