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They significantly increase scale and throughput while reducing costs by allowing the user to pool multiple library preparations into a single sequencing reaction. The AIR Barcoded Adapters enable the user to detect rare sample events amongst hundreds of samples, across several time points and even multiple genomes. Predesigned AIR Barcoded Adapters, are available in sets of 10, 20, or 30 and were designed based on uniform melting temperature (Tm) and sequences that have unique color space. Once attached to a sample, they may be pooled together and sequenced in a single flow cell run. Analysis post run allows the user to identify the sequence barcode back to specific samples.