TATAA Biocenter Scientists Pioneer 3-Way Expression Profiling
News Apr 25, 2008
Taking real-time PCR one step forward researcher at TATAA Biocenter has performed a 3-way expression profiling study of the metabolism of Saccharomyces cerevisiae (baker’s yeast).
While regular (2-way) expression profiling, where a set of samples are analyzed for many genes, is challenging to most researchers, the TATAA scientists analyzed the expression of many (18) genes at 8 different time points after addition of glucose to yeast grown in ethanol of 4 different strains. The experimental design generates a cube of data.
The company said that using GenEx software the data were analyzed using augmented principal component analysis, which revealed important co-regulations between the metabolic genes, including their sensitivities to genetic modifications. This novel 3-way approach to study expression pathways is expected to become very important in studies of potential drug targets, biological responses, and genetically modified species.
Scientists from the UNC School of Medicine discovered that the anti-inflammatory protein NLRP12 normally helps protect mice against obesity and insulin resistance when they are fed a high-fat diet. The researchers also reported that the NLRP12 gene is underactive in people who are obese, making it a potential therapeutic target for treating obesity and diabetes.READ MORE