Tecan Systems Handle High Throughput Prediction of Cancer Recurrence
News Aug 08, 2007
Researchers at the life sciences company Genomic Health Inc., California, have chosen Tecan’s liquid handling workstations to automate the development of their multi-gene expression assay that predicts the risk of recurrence of cancer and the likelihood of chemotherapy benefit in a large portion of early-stage breast cancer patients.
Jay Snable, Director of Process Automation, explained: “We needed automated systems for our ongoing assay development and we chose Tecan for its highly flexible technology. Tecan provided the best solutions for handling all the different formats we need, from single tubes through to 384-well microplates, in a highly integrated system with quality controls all the way.”
Snable continued, “We have developed a gene panel to quantify the likelihood of breast cancer recurrence, and we are currently looking at 761 genes in an initial screen to create a gene profile for an assay to test for risk of recurrence of another cancer type - this is where the Tecan technology will prove especially useful for the high throughput we need to achieve.”
Based on Tecan’s Freedom EVO® 200 and Genesis™ platforms, all assay steps after RNA extraction are automated, from RNA quantification and quality control, reverse transcription and quantitative PCR assay assembly. The workstations are also equipped with a PosID™ System for automated barcode scanning and identification to track individual samples through all the assay steps.
There are hundreds of thousands of structural variants (SVs) in the human genome that are hard to identify than single nucleotide variants. Researchers have used a full suite of genomic technologies to analyze the number of SVs in three individuals, and the results present as the most comprehensive catalog of SVS to date.READ MORE