UBC Scientists Find new Way to Extract Diluted and Contaminated DNA
News Aug 14, 2009
University of British Columbia researchers have developed a new way to extract DNA and RNA from small or heavily contaminated samples that could help forensic investigators and molecular biologists get to “the truth.”
“By exploiting the physical traits of DNA – electric charge, length and flexibility – we’ve been able to extract DNA from samples that would otherwise not produce enough clean DNA for analysis,” says UBC Biophysics Prof. Andre Marziali.
The technique is being commercialized through Boreal Genomics, a UBC spin-off company, and is expected to have broad applications from basic life-science research to forensic sample analysis, bio-defence and pathogen detection for food safety and clinical diagnostics.
The research team, which includes scientists from UBC and BC Cancer Agency’s Genome Science Centre, details the technique in this week’s Proceedings of the National Academy of Science.
Extracting DNA by conventional methods – which rely on the molecules’ chemical properties – has proven challenging when there are only trace amounts of DNA or when the source sample has contaminants with similar chemical traits.
“We’ve found that DNA and RNA respond to electric fields in a way that is very different from other molecules,” says Marziali. “By exploiting this unique property, we were able to extract high quality DNA from a highly contaminated sample from the Athabasca oil sands.”
The team also successfully tested the technique on samples provided by the RCMP.
Schizophrenics' Blood Contains RNA From More MicrobesNews
The blood of schizophrenia patients features genetic material from more types of microorganisms than that of people without the debilitating mental illness, research at Oregon State University has found. What’s not known is whether that’s a cause or effect of the severe, chronic condition that strikes about one person in 100.READ MORE
Faulty Gene Leads to Alcohol-Induced Heart FailureNews
A faulty gene interacts with alcohol to accelerate heart failure in susceptible patients, a study suggests. This dangerous interaction can occur even when only moderate amounts of alcohol have been consumed.READ MORE